Fruit DNA Extraction and Analysis
Fruit DNA extraction and analysis using various techniques
Project Overview
This project aimed to determine the DNA yield of different fruit samples and investigate the influence of growing conditions on DNA concentration. Using ethanol precipitation and the Diphenylamine Method for quantitative DNA estimation, students gained hands-on experience in molecular biology techniques.
Timeline
October 2024 - February 2025
Team
MRT Research Team 2024-2025
Status
Completed & Published
Lab Events Timeline
Info Session
Introduction to the project and methodology. Room A-369 during AP.
Scientific Essay Writing Workshop
With Professor Pierre-Alexandre Mailhot. Room D-120A. Introduction to writing scientific papers using Typst.
Science Fair Prep Session
Executives discussed research projects to help kickstart science fair projects.
Research Challenge
Students helped develop the lab protocol and prepared the standard curve of calf thymus DNA.
DNA Standard Curve and Preparing Chemicals
Preparation of chemicals and establishing standard curves for DNA quantification.
DNA Extraction Lab Project
Main experimental phase where students extracted and analyzed DNA from various fruit samples.
Project Components
Experiment and Manipulations
Students conducted the main experiments and manipulations to obtain results and data for analysis. Due to limited lab space and materials, participation was prioritized for active members.
Writing the Article
Participants analyzed experimental results and crafted various sections of the scientific article. All contributors had their names included in the final published article.
Main Methods
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DNA Extraction Using Ethanol Precipitation
Standard method for isolating DNA from fruit samples using ethanol-based precipitation techniques.
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Quantitative DNA Concentration Estimation
Used the Diphenylamine Method to accurately measure DNA concentration in extracted samples.
Project Resources
Research Challenge
Help us make the lab protocol and get a chance to help make the standard curve of calf thymus DNA
DNA Lab Info Session Slides
October 2024 - Lab protocol briefing and project overview
Lab Project Participation Sheet
Choose what you want to work on for the DNA extraction lab project
Final Report - DNA Analysis Project
Published research article (PDF)